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Development of cytoplasmic male sterile lines in chilli (Capsicum annuum L.) and their evaluation across multiple environments.

ABSTRACT: A breeding program was initiated in 2009 to develop temperature stable CMS lines in chilli. 'CCA 4261' was used as a CMS donor. From the 11 testcross progeny screened, maintainer plants were identified from 'SL 461', 'SL 462' and 'SD 463'. After 6 backcrosses to the maintainer plants, 17 CMS lines in diverse genetic backgrounds were established. The CMS lines were evaluated for stability of sterility over four environments during 2014-15 and 2015-16. The environments E1 and E3 represented the low temperature regime, and E2 and E4 the high temperature regime. The mean square values due to the genotypes and the environments were significant at p = 0.01 for pollen sterility (%), pollen release score, fruit setting (%) and number of seed fruit-1. The G × E interaction effects were significant for pollen sterility (%), fruit setting and number of seed fruit-1 and non-significant for pollen release score. Ten lines namely 'CMS4611A', 'CMS4614A', 'CMS4622A', 'CMS4624A', 'CMS4626A', 'CMS46213A', 'CMS463D2A', 'CMS463D13A', 'CMS463D14A' and 'CMS463L5A' were completely male sterile across the environments. Under open pollination conditions, the fruit and the seed setting ability of these lines was normal. The CMS transferred into the diverse genetic backgrounds would broaden the CMS germplasm resources in chilli.

SUBMITTER: Meena OP

PROVIDER: S-EPMC6198902 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

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Development of cytoplasmic male sterile lines in chilli (Capsicum annuum L.) and their evaluation across multiple environments.

Meena Om Prakash OP Dhaliwal Major Singh MS Jindal Salesh Kumar SK

Breeding science 20180825 4

A breeding program was initiated in 2009 to develop temperature stable CMS lines in chilli. 'CCA 4261' was used as a CMS donor. From the 11 testcross progeny screened, maintainer plants were identified from 'SL 461', 'SL 462' and 'SD 463'. After 6 backcrosses to the maintainer plants, 17 CMS lines in diverse genetic backgrounds were established. The CMS lines were evaluated for stability of sterility over four environments during 2014-15 and 2015-16. The environments E1 and E3</s ...[more]

PMID: 30369814

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Project description:Background: Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives: In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods: Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (Tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results: In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions: We screened 1645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.

Project description:Bacterial blight (BB), caused by Xanthomonas oryzae pv.oryzae is one among the major diseases in rice, which in severe condition cause losses up to 60% in total yield. Marker assisted pyramiding of three broad spectrum BB resistance genes (xa5, xa13, and Xa21) in prominent rice varieties is the most economical and effective strategy for the management of the BB disease. We report here the pyramiding of three genes (xa5, xa13, and Xa21) in maintainer lines (CO 2B, CO 23B, and CO 24B) of three promising wild abortive cytoplasmic male sterile lines (CO 2A, CO 23A, and CO 24A) through functional markers assisted back cross breeding. IRBB60 with xa5, xa13, and Xa21 genes is used as a donor parent. BC2F1 and BC2F2 generations from a cross of CO 2B, CO 23B, and CO 24B with IRBB60 were evaluated for bacterial blight and non-fertility restoration. In BC2F1, plants with all three resistance genes (xa5, xa13, and Xa21) and high parent genome recovery was identified. In BC2F2, plants with all resistance genes and without fertility restorer (Rf3 and Rf4) were selected. Based on agronomic traits, BB resistance and maintenance of sterility, two plants each in CO 2B × IRBB60, CO 24B × IRBB60 and one plant in CO 23B × IRBB60 combinations were identified. The identified lines were crossed with respective male sterile lines for conversion of improved B line into CMS line through back-crossing, in addition to selfing. The plants with high recurrent genome and phenotypically similar to parental lines and sterile are being used for the hybrid rice development program. Currently, using these lines (improved CMS line), test crosses were made to develop new rice hybrids. Hybrids combinations viz., CO 23A × AD08009R and CO 24A × IET20898R were found to be stable at different locations with high yield. The R line used in this study has been introgressed with xa5, xa13, and Xa21 genes in a separate breeding program. These new hybrids with resistance against bacterial blight will increase the crop production at BB environment.

Dataset Information

Development of cytoplasmic male sterile lines in chilli (Capsicum annuum L.) and their evaluation across multiple environments.

Publications

Development of cytoplasmic male sterile lines in chilli (<i>Capsicum annuum</i> L.) and their evaluation across multiple environments.

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