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Protective Effects of 17?-Estradiol on Benzo(e) pyrene[B(e)P]-induced Toxicity in ARPE-19 cells.

ABSTRACT: Purpose:The aim of this study was to examine the effect of 17?-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells. Methods:We pretreated ARPE-19 cells with 20 nM and 40 nM 17?-estradiol for 6 hours, followed by addition of 300 ?M B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (??m). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured. Results:Our results demonstrated that when treated with B(e)P, the viability and ??m of ARPE-19 cells declined by 25% and 63%, respectively (P < 0.05). However, pretreating with 17?-estradiol increased the viability of ARPE-19 cells by 21% (20 nM) and 10% (40 nM) (P < 0.05). Furthermore, the significantly reduced ??m in ?E+B(e)P treated cells ARPE-19 cells was restored by pre-treatment with 17?-estradiol- ??m was increased by 177% (20 nM) and 158% (40 nM) (P < 0.05). We further observed a significant up-regulation in the activity of Caspases-3/7, -9, and -12 in B(e)P-treated ARPE-19 cells. However, 17?-estradiol treatment significantly reduced the activity of all apoptotic markers (P < 0.05). Conclusion:In conclusion, our results demonstrate that 17?-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential.

SUBMITTER: Sapkal AU

PROVIDER: S-EPMC6210882 | biostudies-other | 2018 Oct-Dec

REPOSITORIES: biostudies-other

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Protective Effects of 17β-Estradiol on Benzo(e) pyrene[B(e)P]-induced Toxicity in ARPE-19 cells.

Sapkal Ashish U AU Nashine Sonali S Mansoor Saffar S Sharma Vishal R VR Ramirez Claudio A CA Migon Rafael Z RZ Gupta Navin K NK Chwa Marilyn M Kuppermann Baruch D BD Kenney M Cristina MC

Journal of ophthalmic & vision research 20181001 4

<h4>Purpose</h4>The aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells.<h4>Methods</h4>We pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers ...[more]

PMID: 30479711

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Dataset Information

Protective Effects of 17?-Estradiol on Benzo(e) pyrene[B(e)P]-induced Toxicity in ARPE-19 cells.

Publications

Protective Effects of 17β-Estradiol on Benzo(e) pyrene[B(e)P]-induced Toxicity in ARPE-19 cells.

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