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Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage.


ABSTRACT: Several studies have reported the effects of vitamin C (L-ascorbic acid, AA) on ultraviolet B (UVB)-induced cell damage using cultured keratinocytes. However, the epidermis consists of multiple cell layers, and the effect of AA on UVB-induced damage to the human epidermis remains unclear. Therefore, we investigated the effect of AA on UVB-induced skin damage using reconstituted human epidermis. The reconstituted human epidermal surface was treated with 100 and 500?mM AA and cultured for 3?h before (pre-AA treatment) or after (post-AA treatment) 120?mJ/cm2 UVB irradiation. Pre- and post-AA treatments of the epidermal surface suppressed UVB-induced cell death, apoptosis, DNA damage, reactive oxygen species (ROS) production, and the inflammatory response by downregulating tumour necrosis factor-? (TNF-?) expression and release. Moreover, the pre-AA treatment was more effective at preventing UVB-induced skin damage than the post-AA treatment. In summary, pre- and post-AA treatments of the epidermis prevent UVB-induced damage.

SUBMITTER: Kawashima S 

PROVIDER: S-EPMC6212420 | biostudies-other | 2018 Nov

REPOSITORIES: biostudies-other

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Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage.

Kawashima Saki S   Funakoshi Tomoko T   Sato Yasunori Y   Saito Norikatsu N   Ohsawa Hajime H   Kurita Katsumi K   Nagata Kisaburo K   Yoshida Masayuki M   Ishigami Akihito A  

Scientific reports 20181101 1


Several studies have reported the effects of vitamin C (L-ascorbic acid, AA) on ultraviolet B (UVB)-induced cell damage using cultured keratinocytes. However, the epidermis consists of multiple cell layers, and the effect of AA on UVB-induced damage to the human epidermis remains unclear. Therefore, we investigated the effect of AA on UVB-induced skin damage using reconstituted human epidermis. The reconstituted human epidermal surface was treated with 100 and 500 mM AA and cultured for 3 h befo  ...[more]

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