Ontology highlight
ABSTRACT:
SUBMITTER: Ukah OB
PROVIDER: S-EPMC6212899 | biostudies-other | 2018 Sep
REPOSITORIES: biostudies-other
Ukah Obiaara B OB Puray-Chavez Maritza M Tedbury Philip R PR Herschhorn Alon A Sodroski Joseph G JG Sarafianos Stefan G SG
Viruses 20180930 10
We have recently developed the first microscopy-based strategy that enables simultaneous multiplex detection of viral RNA (vRNA), viral DNA (vDNA), and viral protein. Here, we used this approach to study the kinetics of latency reactivation in cells infected with the human immunodeficiency virus (HIV). We showed the transcription of nascent vRNA from individual latently integrated and reactivated vDNA sites appearing earlier than viral protein. We further demonstrated that this method can be use ...[more]