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High-throughput multiplexed fluorescence-activated droplet sorting.

ABSTRACT: Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.

SUBMITTER: Caen O

PROVIDER: S-EPMC6220162 | biostudies-other | 2018

REPOSITORIES: biostudies-other

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High-throughput multiplexed fluorescence-activated droplet sorting.

Caen Ouriel O Schütz Simon S Jammalamadaka M S Suryateja MSS Vrignon Jérémy J Nizard Philippe P Schneider Tobias M TM Baret Jean-Christophe JC Taly Valérie V

Microsystems & nanoengineering 20181022

Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to sep ...[more]

PMID: 31057921

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