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Design and modeling of pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye.

ABSTRACT: A pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye is proposed. A diffractive optical element is used to enlarge field of view (FOV) of transmitting system and a receiving system consisting of a non-uniform microlens array, an aperture array, and an avalanche photodiode array is designed. The non-uniform microlens array is arranged on a curved surface to mimic large FOV feature of the compound eye. Meanwhile, the non-uniform microlens array is modeled to mimic space-variant resolution property of the human eye. On the basis of the proposed system, some simulation experiments are carried out. Results show that the entire FOV is up to 52°, and the resolution is 30?×?18. The proposed system has a high resolution in the center FOV and a low resolution in the peripheral FOV. The rotation and scaling invariances of the human eye are verified on the proposed system. The signal-to-noise ratio (SNR) increases with the increase in the number of rings and the maximum SNR locates at the outmost periphery area. This work is beneficial to the design of the pulsed laser three-dimensional imaging system with large FOV, high speed, and high resolution.

SUBMITTER: Cheng Y

PROVIDER: S-EPMC6249249 | biostudies-other | 2018 Nov

REPOSITORIES: biostudies-other

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Design and modeling of pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye.

Cheng Yang Y Cao Jie J Zhang Fanghua F Hao Qun Q

Scientific reports 20181121 1

A pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye is proposed. A diffractive optical element is used to enlarge field of view (FOV) of transmitting system and a receiving system consisting of a non-uniform microlens array, an aperture array, and an avalanche photodiode array is designed. The non-uniform microlens array is arranged on a curved surface to mimic large FOV feature of the compound eye. Meanwhile, the non-uniform microlens array is modeled to mi ...[more]

PMID: 30464304

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Project description:Targeted intracellular delivery of biomolecules and therapeutic cargo enables the controlled manipulation of cellular processes. Laser-based optoporation has emerged as a versatile, non-invasive technique that employs light-based transient physical disruption of the cell membrane and achieves high transfection efficiency with low cell damage. Testing of the delivery efficiency of optoporation-based techniques has been conducted on single cells in monolayers, but its applicability in three-dimensional (3D) cell clusters/spheroids has not been explored. Cancer cells grown as 3D tumor spheroids are widely used in anti-cancer drug screening and can be potentially employed for testing delivery efficiency. Towards this goal, we demonstrated the optoporation-based high-throughput intracellular delivery of a model fluorescent cargo (propidium iodide, PI) within 3D SiHa human cervical cancer spheroids. To enable this technique, nano-spiked core-shell gold-coated polystyrene nanoparticles (ns-AuNPs) with a high surface-to-volume ratio were fabricated. ns-AuNPs exhibited high electric field enhancement and highly localized heating at an excitation wavelength of 680 nm. ns-AuNPs were co-incubated with cancer cells within hanging droplets to enable the rapid aggregation and assembly of spheroids. Nanosecond pulsed-laser excitation at the optimized values of laser fluence (45 mJ cm-2), pulse frequency (10 Hz), laser exposure time (30 s), and ns-AuNP concentration (5 × 1010 particles per ml) resulted in the successful delivery of PI dye into cancer cells. This technique ensured high delivery efficiency (89.6 ± 2.8%) while maintaining high cellular viability (97.4 ± 0.4%), thereby validating the applicability of this technique for intracellular delivery. The optoporation-based strategy can enable high-throughput single cell manipulation, is scalable towards larger 3D tissue constructs, and may provide translational benefits for the delivery of anti-cancer therapeutics to tumors.

Dataset Information

Design and modeling of pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye.

Publications

Design and modeling of pulsed-laser three-dimensional imaging system inspired by compound and human hybrid eye.

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