Dataset Information

Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.

ABSTRACT: To understand the burgeoning challenges of metastasis, a microchannel of 35 ?m diameter, constricted to 7 ?m for a distance of 200 ?m in a total length of 3?mm, was designed and fabricated using a mask aligner made of polydimethylsiloxane (PDMS) to mimic in vivo capillaries. A thin glass cover-slide was mounted on top to monitor the motion of single or aggregated malignant HeLa cells (size 17-30 ?m) microscopically through the constricted microchannel at a constant flow rate of 30??l/h. Quantitative deconvolution of high-speed videographs of a single cell of 30 ?m revealed cellular deformation while passing through constriction, having elongation index, average transit velocity and entry time of 2.67, 18?mm/s and 5.1?ms, respectively. Morphological analysis of live and apoptotic cells by dual staining with Acridine Orange/Ethidium Bromide demonstrated retention of a significant viable cell population after exit through the constriction and a viability index of 50% was quantified by dye exclusion assay. The cumulative data for microfluidic parameters, morphology and relevant metastatic MMP2 gene expression efficiency measured by real-time polymerase chain reaction revealed retention of virulence potency that could possibly cause metastasis, would be beneficial in developing futuristic MEMS device for cancer theranostics.

SUBMITTER: Nath B

PROVIDER: S-EPMC6255798 | biostudies-other | 2018 Nov

REPOSITORIES: biostudies-other

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Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.

Nath Binita B Raza Asif A Sethi Vishal V Dalal Amaresh A Ghosh Siddhartha Sankar SS Biswas Gautam G

Scientific reports 20181126 1

To understand the burgeoning challenges of metastasis, a microchannel of 35 μm diameter, constricted to 7 μm for a distance of 200 μm in a total length of 3 mm, was designed and fabricated using a mask aligner made of polydimethylsiloxane (PDMS) to mimic in vivo capillaries. A thin glass cover-slide was mounted on top to monitor the motion of single or aggregated malignant HeLa cells (size 17-30 μm) microscopically through the constricted microchannel at a constant flow rate of 30 μl/h. Quantita ...[more]

PMID: 30478455

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Dataset Information

Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.

Publications

Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.

Similar Datasets

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