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Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation.

ABSTRACT: Clathrin light chains (CLCs) control selective uptake of a range of G protein-coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y12, but it is dispensable for the constitutive uptake of the transferrin receptor (TfR). We demonstrate that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the transition of flat lattices into invaginated buds. This transition is dependent on efficient clathrin exchange regulated by CLCb phosphorylation and mediated through auxilin. Strikingly, this rearrangement is required for the uptake of P2Y12 but not TfR. These findings link auxilin-mediated clathrin exchange to early stages of CCP invagination in a cargo-specific manner. This supports a model in which CCPs invaginate with variable modes of curvature depending on the cargo they incorporate.

SUBMITTER: Maib H

PROVIDER: S-EPMC6279376 | biostudies-other | 2018 Dec

REPOSITORIES: biostudies-other

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Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation.

Maib Hannes H Ferreira Filipe F Vassilopoulos Stéphane S Smythe Elizabeth E

The Journal of cell biology 20180918 12

Clathrin light chains (CLCs) control selective uptake of a range of G protein-coupled receptors (GPCRs), although the mechanism by which this occurs has remained elusive thus far. In particular, site-specific phosphorylation of CLCb controls the uptake of the purinergic GPCR P2Y<sub>12</sub>, but it is dispensable for the constitutive uptake of the transferrin receptor (TfR). We demonstrate that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the tra ...[more]

PMID: 30228161

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