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Cell migration–inducing hyaluronan-binding protein is regulated by miR-140-3p and promotes the growth and invasion of colorectal cancer cells.

ABSTRACT: Dysregulation of cell migration–inducing hyaluronan-binding protein (CEMIP) is associated with the growth and metastasis of multiple malignancies. But, the underlying mechanism by which CEMIP contributes to colorectal cancer (CRC) remains undocumented. The association of CEMIP or miR-140-3p expression with clinicopathological characteristics and prognosis in CRC patients was analyzed by the tissue microarray and TCGA dataset. MiR-140-3p-specific binding with CEMIP was confirmed by luciferase report assay. In vitro experiments were conducted to assess the effects of CEMIP on the growth and invasion of CRC cells. Consequently, we found that CEMIP expression was dramatically elevated in CRC tissues and associated with a poor prognosis in CRC patients. The upregulation of CEMIP was attributable to the dysregulation of miR-140-3p rather than its genetic and epigenetic alterations. Ectopic expression of CEMIP facilitated the cell viability, colony formation, and invasive potential, but silencing of CEMIP reversed these effects. Furthermore, CEMIP was identified as a direct target of miR-140-3p and attenuated miR-140-3p-induced anti-proliferation effects by regulating c-Myc, E-cadherin, and Twist-1 expression. MiR-140-3p indicated a negative correlation with CEMIP expression and was an independent prognostic factor of tumor recurrence in CRC patients. Taken together, CEMIP is regulated by miR-140-3p and promotes the growth and invasion of CRC cells. MiR-140-3p/CEMIP axis may represent the potential markers for CRC patients.

SUBMITTER: Yang X

PROVIDER: S-EPMC6291868 | biostudies-other | 2018 Mar-Dec

REPOSITORIES: biostudies-other

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Project description:BackgroundThe present study is aimed at exploring the specific expression of miR-193a-3p and the mechanism underlying miR-193a-3p-mediated mesenchymal transition (MT), invasion, and migration in glioma.MethodsThe gene expression profile datasets of GSE39486 and GSE25676 were downloaded from the National Center for Biotechnology (NCBI). Data regarding the expression of miR-193a-3p and survival curves were derived from Chinese Glioma Genome Atlas (CGGA). Online websites including miRWalk, DIANA, and starbase were employed to predict the target genes for miR-193a-3p. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by the Omicsbean online software. Module analysis of the protein-protein interaction (PPI) networks was performed by the plug-in Molecular Complex Detection (MCODE), and the degrees of genes were calculated by CytoHubba plug-in of Cytoscape. Survival curves were based on the Gene Expression Profile Interaction Analysis (GEPIA). Transwell, wound healing, and Western blot experiments were performed to investigate the effects of miR-193a-3p and beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) on the invasion, migration, and MT of glioma.ResultsmiR-193a-3p was highly expressed in glioma tissues and significantly correlated with poor survival in patients with glioma. The target genes for miR-193a-3p were involved in many cancer-related signaling pathways. The PPI showed 11 genes with both high degrees and MCODE scores in the network. Survival analysis demonstrated that the expression of BTRC was significantly correlated with the prognosis of patients with glioma. The results from the transwell, wound healing, and Western blot analyses suggested that miR-193a-3p promoted the invasion, migration, and MT of glioma cells, which could be reversed by BTRC.ConclusionsmiR-193a-3p was upregulated in patients with glioma and could affect the invasion, migration, and MT of glioma by regulating BTRC.

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Cell migration–inducing hyaluronan-binding protein is regulated by miR-140-3p and promotes the growth and invasion of colorectal cancer cells.

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