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F-doped TiO2 microporous coating on titanium with enhanced antibacterial and osteogenic activities.

ABSTRACT: To enhance bacterial resistance and osteogenesis of titanium (Ti) -based implants, TiO2/calcium-phosphate coatings (TiCP) doped with various amounts of fluorine (F) (designated as TiCP-F1, TiCP-F6, and TiCP-F9) were prepared on Ti by micro-arc oxidation. The F doped TiCP coatings possess a microporous structure (pore size of 3-4 ?m in average diameter) which is evenly covered by nano-grains of 30-60?nm in size. Successful F incorporation into TiCP was determined by X-ray photoelectron spectroscopy, and it shows weak influence on the microstructure, phase compositions, surface roughness and wettability of TiCP. All the coatings bonded firmly to the Ti substrates and showed enduring high adhesion strength in biological circumstances. The bacterial resistance and osteogenesis of the coatings were evaluated by implanting testing materials in vitro and in an infected rabbit model caused by bacteria. Both the in vitro and in vivo results indicated that TiCP and TiCP-F1 were of much higher osteogenic activity compared with Ti but lacking of bacterial resistance, whereas TiCP with high F addition (TiCP-F6 and TiCP-F9) exhibited both dramatically improved bacterial resistance and osteogenesis. In summary, TiCP-F6 possessed the best antibacterial and osteogenic activities, especially exhibited excellent osseointegration efficacy in the infected rabbit model.

SUBMITTER: Zhou J

PROVIDER: S-EPMC6294799 | biostudies-other | 2018 Dec

REPOSITORIES: biostudies-other

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F-doped TiO<sub>2</sub> microporous coating on titanium with enhanced antibacterial and osteogenic activities.

Zhou Jianhong J Li Bo B Han Yong Y

Scientific reports 20181214 1

To enhance bacterial resistance and osteogenesis of titanium (Ti) -based implants, TiO<sub>2</sub>/calcium-phosphate coatings (TiCP) doped with various amounts of fluorine (F) (designated as TiCP-F1, TiCP-F6, and TiCP-F9) were prepared on Ti by micro-arc oxidation. The F doped TiCP coatings possess a microporous structure (pore size of 3-4 μm in average diameter) which is evenly covered by nano-grains of 30-60 nm in size. Successful F incorporation into TiCP was determined by X-ray photoelectron ...[more]

PMID: 30552353

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Project description:BACKGROUND:The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. METHODS:Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. RESULTS:The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. CONCLUSIONS:The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

Project description:To research the relationship of micro-structures and antibacterial properties of the titanium-doped ZnO powders and probe their antibacterial mechanism, titanium-doped ZnO powders with different shapes and sizes were prepared from different zinc salts by alcohothermal method. The ZnO powders were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED), and the antibacterial activities of titanium-doped ZnO powders on Escherichia coli and Staphylococcus aureus were evaluated. Furthermore, the tested strains were characterized by SEM, and the electrical conductance variation trend of the bacterial suspension was characterized. The results indicate that the morphologies of the powders are different due to preparation from different zinc salts. The XRD results manifest that the samples synthesized from zinc acetate, zinc nitrate, and zinc chloride are zincite ZnO, and the sample synthesized from zinc sulfate is the mixture of ZnO, ZnTiO3, and ZnSO4 · 3Zn (OH)2 crystal. UV-vis spectra show that the absorption edges of the titanium-doped ZnO powders are red shifted to more than 400 nm which are prepared from zinc acetate, zinc nitrate, and zinc chloride. The antibacterial activity of titanium-doped ZnO powders synthesized from zinc chloride is optimal, and its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are lower than 0.25 g L-1. Likewise, when the bacteria are treated by ZnO powders synthesized from zinc chloride, the bacterial cells are damaged most seriously, and the electrical conductance increment of bacterial suspension is slightly high. It can be inferred that the antibacterial properties of the titanium-doped ZnO powders are relevant to the microstructure, particle size, and the crystal. The powders can damage the cell walls; thus, the electrolyte is leaked from cells.

Dataset Information

F-doped TiO2 microporous coating on titanium with enhanced antibacterial and osteogenic activities.

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F-doped TiO<sub>2</sub> microporous coating on titanium with enhanced antibacterial and osteogenic activities.

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