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A simple in vitro method to evaluate the toxicity of functional additives used in shrimp aquaculture.

ABSTRACT: To mitigate the economic losses provoked by disease outbreaks, shrimp producers employ therapeutic additives. However, important issues such as the toxicity of these products on shrimp are not always considered. In vivo toxicity assays require a lot of time and large economic and physical resources. Here, we describe an in vitro procedure to evaluate the toxicity of functional additives, used in the production of shrimp Penaeus vannamei. This method adapted the cell viability assay based on the reduction of tetrazolium salts (MTT) to primary cell cultures of shrimp hemocytes. •A simple and reliable tool that requires few physical and economic resources to evaluate in short time (6?h) the cytotoxic effect of therapeutic products and additives to be included in shrimp culture•This inexpensive method requires only a modified Hank's balanced salt solution (HBSS) containing Ca2+ and Mg2+ to keep hemocytes metabolically active to successfully carry out the cytotoxicity assay•This toxicity in vitro assay does not require exposure of the shrimp to compounds at toxic concentrations.

SUBMITTER: Dominguez-Borbor C

PROVIDER: S-EPMC6314867 | biostudies-other | 2018

REPOSITORIES: biostudies-other

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A simple in vitro method to evaluate the toxicity of functional additives used in shrimp aquaculture.

Domínguez-Borbor Cristóbal C Chalén-Alvarado Bolívar B Rodríguez Jenny A JA

MethodsX 20180131

To mitigate the economic losses provoked by disease outbreaks, shrimp producers employ therapeutic additives. However, important issues such as the toxicity of these products on shrimp are not always considered. In vivo toxicity assays require a lot of time and large economic and physical resources. Here, we describe an in vitro procedure to evaluate the toxicity of functional additives, used in the production of shrimp Penaeus vannamei. This method adapted the cell viabilit ...[more]

PMID: 30619722

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Project description:Detailed geochemical studies of both major and minor elements in Bangladesh surface waters are sparse, particularly in shrimp aquaculture pond environments. Therefore, water samples from shrimp aquaculture ponds and tidal channels were collected in high precipitation (July) and low precipitation (May) months from 2018-2019 in Southwest Bangladesh and analyzed for complete water chemistry. Selenium (Se) and arsenic (As) were elevated above WHO guidelines in 50% and ~ 87% of samples, respectively, but do not show any recognizable spatial patterns. Shrimp pond and tidal channel water compositions in the dry season (May) are similar, illustrating their connectivity and minimal endogenous effects within shrimp ponds. Tidal channels are less saline in July than shrimp ponds still irrigated by tidal channels, suggesting that either farmers limit irrigation to continue farming saltwater shrimp, or the irrigation flux is low and leads to a lag in aquaculture-tidal channel compositional homogenization. δ18O and δ2H isotopic compositions from samples in May of 2019 reveal tidal channel samples are closer to the local meteoric water line (LMWL) than shrimp pond samples, because of less evaporation. However, evaporation in May shrimp ponds has a minimal effect on water composition, likely because of regular drainage/exchange of pond waters. Dissolved organic carbon (DOC) is positively correlated with both δ18O and δ2H in shrimp ponds, suggesting that as evaporation increases, DOC becomes enriched. Multiple linear regression reveals that As and Se can be moderately predicted (adjusted R2 values between 0.4 and 0.7, p < 0.01) in surface waters of our study with only 3-4 independent predictor variables (e.g., Ni, V and DOC for Se prediction; Cu, V, Ni and P for As prediction). Thus, this general approach should be followed in other regions throughout the world when measurements for certain hazardous trace elements such as Se and As may be lacking in several samples from a dataset.

Dataset Information

A simple in vitro method to evaluate the toxicity of functional additives used in shrimp aquaculture.

Publications

A simple <i>in vitro</i> method to evaluate the toxicity of functional additives used in shrimp aquaculture.

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