Unknown

Dataset Information

0

A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H?O? in Human Embryonic Kidney (HEK) 293T Cells.


ABSTRACT: Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H?O?-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H?O?-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H?O?. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H?O?-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.

SUBMITTER: Xu N 

PROVIDER: S-EPMC6320779 | biostudies-other | 2018 Dec

REPOSITORIES: biostudies-other

altmetric image

Publications

A Polysaccharide Purified from <i>Morchella conica</i> <i>Pers.</i> Prevents Oxidative Stress Induced by H₂O₂ in Human Embryonic Kidney (HEK) 293T Cells.

Xu Na N   Lu Yi Y   Hou Jumin J   Liu Chao C   Sun Yonghai Y  

International journal of molecular sciences 20181213 12


<i>Morchella conica Pers.</i> (<i>M. conica</i>) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from <i>M. conica</i> have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral <i>M. conica</i> polysaccharides-2) purified from <i>M. conica,</i> as determined by radical scavenging assay  ...[more]

Similar Datasets

| S-EPMC5524341 | biostudies-other
| S-EPMC7462251 | biostudies-literature
| S-EPMC10341561 | biostudies-literature
| S-EPMC4658483 | biostudies-other
| S-EPMC4742120 | biostudies-literature
| S-EPMC9675453 | biostudies-literature
| S-EPMC7222193 | biostudies-literature
| S-EPMC7057716 | biostudies-literature
| S-EPMC8026309 | biostudies-literature
| S-EPMC5561916 | biostudies-other