MiR-196a-5p promotes metastasis of colorectal cancer via targeting I?B?.

ABSTRACT: BACKGROUND:MicroRNA-196a-5p (miR-196a-5p) has been reported to be involved in the metastatic process of several cancers. In present work, we aimed to investigate the effects of miR-196a-5p and its potential target I?B? on migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells. METHODS:CCK-8 assay, wound healing assay and cell invasion assay were performed to evaluate the cell proliferation, migration and invasion. In vivo metastasis models were used to investigate the tumor metastasis ability. Real-time PCR, immunofluorescence staining or western blot were utilized to detect the expression of miR-196a-5p, I?B?, p-I?B?, nuclear p65 and EMT markers including E-cadherin, N-cadherin and fibronectin. Dual luciferase reporter assay was carried out to determine whether there is a direct interaction between miR-196a-5p and I?B? mRNA. RESULTS:Using SW480 cell with miR-196-5p over-expressed plus SW620 and HCT116 cells with miR-196a-5p knockdown, we found that miR-196a-5p promoted cell proliferation, migration and invasion in vitro and facilitated liver metastasis in vivo. We also observed that miR-196a-5p knockdown or NF-?B pathway inhibition up-regulated E-cadherin while down-regulated N-cadherin and fibronectin. By contrast, miR-196a-5p over-expression promoted EMT process of CRC. Data of dual luciferase reporter assay indicated that miR-196a-5p targeted the I?B?. Moreover, miR-196a-5p down-regulated I?B? expression while up-regulated nuclear p65 expression. Additionally, over-expression of I?B? in CRC cells attenuated the effects of miR-196a-5p on cell migration, invasion and EMT. CONCLUSIONS:miR-196a-5p may play a key role in EMT, invasion and metastasis of CRC cells via targeting the I?B?.

SUBMITTER: Xin H 

PROVIDER: S-EPMC6325824 | biostudies-other | 2019 Jan

REPOSITORIES: biostudies-other