Ontology highlight
ABSTRACT:
SUBMITTER: Mayle R
PROVIDER: S-EPMC6338834 | biostudies-other | 2019 Jan
REPOSITORIES: biostudies-other
Mayle Ryan R Langston Lance L Molloy Kelly R KR Zhang Dan D Chait Brian T BT O'Donnell Michael E ME
Proceedings of the National Academy of Sciences of the United States of America 20181231 3
The 11-subunit eukaryotic replicative helicase CMG (Cdc45, Mcm2-7, GINS) tightly binds Mcm10, an essential replication protein in all eukaryotes. Here we show that Mcm10 has a potent strand-annealing activity both alone and in complex with CMG. CMG-Mcm10 unwinds and then reanneals single strands soon after they have been unwound in vitro. Given the DNA damage and replisome instability associated with loss of Mcm10 function, we examined the effect of Mcm10 on fork regression. Fork regression requ ...[more]