Ontology highlight
ABSTRACT:
SUBMITTER: Rainey KH
PROVIDER: S-EPMC6338835 | biostudies-other | 2019 Jan
REPOSITORIES: biostudies-other
Rainey Kristin H KH Patterson George H GH
Proceedings of the National Academy of Sciences of the United States of America 20181231 3
FRET is a powerful approach to study the interactions of fluorescent molecules, and numerous methods have been developed to measure FRET in cells. Here, we present a method based on a donor molecule's photoswitching properties, which are slower in the presence vs. the absence of an acceptor. The technique, photoswitching FRET (psFRET), is similar to an established but underutilized method called photobleaching FRET (pbFRET), with the major difference being that the molecules are switched "off" r ...[more]