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The effect of 16S rRNA region choice on bacterial community metabarcoding results.


ABSTRACT: In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.

SUBMITTER: Bukin YS 

PROVIDER: S-EPMC6362892 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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The effect of 16S rRNA region choice on bacterial community metabarcoding results.

Bukin Yu S YS   Galachyants Yu P YP   Morozov I V IV   Bukin S V SV   Zakharenko A S AS   Zemskaya T I TI  

Scientific data 20190205


In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered b  ...[more]

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