The effect of 16S rRNA region choice on bacterial community metabarcoding results.
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ABSTRACT:
SUBMITTER: Bukin YS
PROVIDER: S-EPMC6362892 | biostudies-other | 2019 Feb
REPOSITORIES: biostudies-other
Publications
The effect of 16S rRNA region choice on bacterial community metabarcoding results.
Bukin Yu S YS Galachyants Yu P YP Morozov I V IV Bukin S V SV Zakharenko A S AS Zemskaya T I TI
Scientific data 20190205
In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered b ...[more]
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