Dataset Information

Hexanal, a major volatile found in fresh peanut seed, elicits foraging behavior in the laboratory-reared brown marmorated stink bug, Halyomorpha halys (Heteroptera: Pentatomidae).

ABSTRACT: Phytophagous insects utilize visual, olfactory and gustatory cues to find food. The brown marmorated stink bug, Halyomorpha halys (Stål), quickly approaches fresh peanut seeds newly introduced into the rearing cage in the laboratory but shows less interest in stale peanut seed previously infested by conspecifics. This observation suggests that H. halys can perceive the quality of food by detecting the volatile(s) from fresh peanut seeds. A bioassay revealed that H. halys adults could more quickly find fresh peanut seeds than three-day-infested peanut seeds, which is consistent with laboratory observations. Hexanal was found to be the major volatile component of fresh peanut seeds but not of previously infested ones. In the two-choice assays, the adult bugs that did respond were significantly attracted to fresh peanut volatiles and hexanal. Hexanal also induced proboscis-protruding behavior in adult H. halys, which suggested that this compound is a key stimulant of foraging behavior of laboratory-reared H. halys adults.

SUBMITTER: Noge K

PROVIDER: S-EPMC6389837 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Hexanal, a major volatile found in fresh peanut seed, elicits foraging behavior in the laboratory-reared brown marmorated stink bug, Halyomorpha halys (Heteroptera: Pentatomidae).

Noge Koji K

Journal of pesticide science 20190201 1

Phytophagous insects utilize visual, olfactory and gustatory cues to find food. The brown marmorated stink bug, Halyomorpha halys (Stål), quickly approaches fresh peanut seeds newly introduced into the rearing cage in the laboratory but shows less interest in stale peanut seed previously infested by conspecifics. This observation suggests that H. halys can perceive the quality of food by detecting the volatile(s) from fresh peanut seeds. A bioassay revealed that H. <i ...[more]

PMID: 30820168

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Project description:Halyomorpha halys (Stål) (Heteroptera: Pentatomidae), the brown marmorated stink bug, is an invasive agricultural and nuisance pest rapidly expanding its incidence in North America. This voracious pest poses a significant threat to rural and urban agriculture, especially to specialty crops such as apples, grapes and ornamentals, as well as staple crops including soybean and corn. The object of this study was to generate transcript sequence resources for H. halys. RNA-seq libraries derived from distinct developmental stages and sexes were sequenced and assembled into 248,569 putatively unique transcripts (PUTs). PUTs were segmented into three disjoint tiers of varying reliability, with 4,794 classified as gold tier (highest quality), 16,878 as silver, and 14,357 as bronze. The gold-tier PUTs associated with 2,580 distinct non-redundant protein sequences from the NCBI NR database--1,785 of these (69%) mapped to annotated UniProtKB database proteins, from which 1,273 unique Pfam families and 459 unique Molecular Function GO terms were encountered. Of the silver tier's 6,527 PUTs associated with unique proteins, 4,193 mapped to UniProtKB (64%), from which 1,941 and 640 unique Pfam and Molecular Function GO terms were extracted. H. halys PUTs related to important life processes like immunity, endocrinology, reproduction, development, behavior, neurotransmission, neurotoxicity, olfaction, and small RNA pathways were validated through quantitative Real-Time PCR (qRT-PCR) for differential expression during distinct life stages (eggs, 2nd instar nymphs, 4th instar nymphs, female adults, male adults). PUTs similar to hypothetical proteins identified in symbiont microbes, including Pantoea and Nosema species, were more abundantly expressed in adults versus nymphs. These comprehensive H. halys transcriptomic resources can be utilized to aid development of novel control methodologies to disrupt life processes; to conduct reverse genetic screens to determine host gene function; and to design environmentally unobtrusive means to control host populations or target specific H. halys life stages, such as molecular biopesticides.

Project description:Triatomine vectors transmit Trypanosoma cruzi, the etiological agent of Chagas disease in humans. Transmission to humans typically occurs when contaminated triatomine feces come in contact with the bite site or mucosal membranes. In the Southern Cone of South America, where the highest burden of disease exists, Triatoma infestans is the principal vector for T. cruzi. Recent studies of other vector-borne illnesses have shown that arthropod microbiota influences the ability of infectious agents to colonize the insect vector and transmit to the human host. This has garnered attention as a potential control strategy against T. cruzi, as vector control is the main tool of Chagas disease prevention. Here we characterized the microbiota in T. infestans feces of both wild-caught and laboratory-reared insects and examined the relationship between microbial composition and T. cruzi infection using highly sensitive high-throughput sequencing technology to sequence the V3-V4 region of the 16S ribosomal RNA gene on the MiSeq Illumina platform. We collected 59 wild (9 with T. cruzi infection) and 10 lab-reared T. infestans (4 with T. cruzi infection) from the endemic area of Arequipa, Perú. Wild T. infestans had greater hindgut bacterial diversity than laboratory-reared bugs. Microbiota of lab insects comprised a subset of those identified in their wild counterparts, with 96 of the total 124 genera also observed in laboratory-reared insects. Among wild insects, variation in bacterial composition was observed, but time and location of collection and development stage did not explain this variation. T. cruzi infection in lab insects did not affect α- or β-diversity; however, we did find that the β-diversity of wild insects differed if they were infected with T. cruzi and identified 10 specific taxa that had significantly different relative abundances in infected vs. uninfected wild T. infestans (Bosea, Mesorhizobium, Dietzia, and Cupriavidus were underrepresented in infected bugs; Sporosarcina, an unclassified genus of Porphyromonadaceae, Nestenrenkonia, Alkalibacterium, Peptoniphilus, Marinilactibacillus were overrepresented in infected bugs). Our findings suggest that T. cruzi infection is associated with the microbiota of T. infestans and that inferring the microbiota of wild T. infestans may not be possible through sampling of T. infestans reared in the insectary.

Dataset Information

Hexanal, a major volatile found in fresh peanut seed, elicits foraging behavior in the laboratory-reared brown marmorated stink bug, Halyomorpha halys (Heteroptera: Pentatomidae).

Publications

Hexanal, a major volatile found in fresh peanut seed, elicits foraging behavior in the laboratory-reared brown marmorated stink bug, <i>Halyomorpha halys</i> (Heteroptera: Pentatomidae).

Similar Datasets

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