Ontology highlight
ABSTRACT:
SUBMITTER: Ali H
PROVIDER: S-EPMC6389893 | biostudies-other | 2019 Feb
REPOSITORIES: biostudies-other
Ali Hashim H Mano Miguel M Braga Luca L Naseem Asma A Marini Bruna B Vu Diem My DM Collesi Chiara C Meroni Germana G Lusic Marina M Giacca Mauro M
Nature communications 20190225 1
Productive HIV-1 replication requires viral integrase (IN), which catalyzes integration of the viral genome into the host cell DNA. IN, however, is short lived and is rapidly degraded by the host ubiquitin-proteasome system. To identify the cellular factors responsible for HIV-1 IN degradation, we performed a targeted RNAi screen using a library of siRNAs against all components of the ubiquitin-conjugation machinery using high-content microscopy. Here we report that the E3 RING ligase TRIM33 is ...[more]