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Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel.

ABSTRACT: Here we show that the well-known ovalbumin epitope SIINFEKL that is routinely used to stimulate ovalbumin-specific T cells and to test new vaccine adjuvants can form a stable hydrogel. We investigate properties of this hydrogel by a range of spectroscopic and imaging techniques demonstrating that the hydrogel is stabilized by self-assembly of the peptide into nanofibres via stacking of β-sheets. As peptide hydrogels are known to stimulate an immune response as adjuvants, the immunoactive properties of the SIINFEKL peptide may also originate from its propensity to self-assemble into a hydrogel. This finding requires a re-evaluation of this epitope in adjuvant testing.

SUBMITTER: Kamalov M

PROVIDER: S-EPMC6390181 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel.

Kamalov Meder M Kählig Hanspeter H Rentenberger Christian C Müllner Alexander R M ARM Peterlik Herwig H Becker Christian F W CFW

Scientific reports 20190225 1

Here we show that the well-known ovalbumin epitope SIINFEKL that is routinely used to stimulate ovalbumin-specific T cells and to test new vaccine adjuvants can form a stable hydrogel. We investigate properties of this hydrogel by a range of spectroscopic and imaging techniques demonstrating that the hydrogel is stabilized by self-assembly of the peptide into nanofibres via stacking of β-sheets. As peptide hydrogels are known to stimulate an immune response as adjuvants, the immunoactive propert ...[more]

PMID: 30804439

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