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Purification of total flavonoids from Rhizoma Smilacis Glabrae through cyclodextrin-assisted extraction and resin adsorption.

ABSTRACT: Flavonoids are the main bioactive components responded for the health promoting effects of Rhizoma Smilacis Glabrae (RSG), an herbal material used in many functional food of China. An eco-friendly method with β-cyclodextrin (β-CD)-assisted extraction and resin adsorption/desorption was developed for total flavonoids purification from RSG. Because of complexes formation between flavonoids and β-CD, aqueous solution was used for extraction instead of ethanol. The CD-assisted extraction was optimized through defining optimal CD species, concentration, extraction temperature, and time. The adsorption property of eight macroporous resins on astilbin was compared by adsorption kinetics and capacity. All resins could reach the adsorption equilibrium within 2 hr. Further analyzed by Langmuir and Freundlich models, H103 resin with the best adsorption capacity was selected. The desorption property of different ethanol-water solution was compared. Results showed that by using 75% ethanol, astilbin could be well desorbed from the resin with the recovery of 96.3%. Because of complexes formation, the presence of β-CD would slightly decrease the adsorption rate and capacity of H103 with concentration dependent. In dynamic adsorption, decreasing the flow rate could minish the effects of β-CD. The developed method was successfully used for total flavonoids purified from RSG. The yield of purified product was 8.78%, with astilbin and total flavonoids content of 363.8 and 505.7 mg/g, respectively. The purity was 1.74 times increased with the recovery of 94.38% compared with the extract obtained directly through 50% ethanol extraction.

SUBMITTER: Zhang L

PROVIDER: S-EPMC6392876 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Purification of total flavonoids from Rhizoma Smilacis Glabrae through cyclodextrin-assisted extraction and resin adsorption.

Zhang Li L Zheng Dan D Zhang Qing-Feng QF

Food science & nutrition 20190129 2

Flavonoids are the main bioactive components responded for the health promoting effects of Rhizoma Smilacis Glabrae (RSG), an herbal material used in many functional food of China. An eco-friendly method with β-cyclodextrin (β-CD)-assisted extraction and resin adsorption/desorption was developed for total flavonoids purification from RSG. Because of complexes formation between flavonoids and β-CD, aqueous solution was used for extraction instead of ethanol. The CD-assisted e ...[more]

PMID: 30847122

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Project description:Nymphaea lotus L. is the medicinal plant that has long been used for food, cosmetics and traditional medicines in Africa and Asia since ancient times. Its flavonoids and other interesting phytochemical compounds from rhizome, leaf and the whole flowers have been reported in the previous published research. However, stamens, which are essential for reproductive functions, may also represent new alternative sources of potential antioxidant flavonoids, as investigated in this study. The innovative green chemistry methods, i.e., ultrasound-assisted extraction (UAE) as well as a macroporous resin (MPR) purification procedure, were employed in this current research. Using a full factorial design coupled to three-dimensional (3D) surface plot methodology, the influence of three variables, namely aqEtOH concentration (ranging from 50 to 100% (v/v), US frequency (ranging from 0 (no US applied) to 45 kHz), and the extraction duration (ranging from 20 to 60 min), were evaluated. Five MPRs with different surface areas, average pore diameters, matrix types and polarities were also investigated for the purification of total flavonoids. The optimal UAE condition is 90% (v/v) aqEtOH with 34.65 khz ultrasonic frequency and 46 min of extraction duration. Compared with the conventional heat reflux extraction (HRE) method, a significant 1.35-fold increase in total flavonoids content was obtained using optimized UAE conditions (169.64 for HRE vs. 235.45 mg/g dry weight for UAE), causing a 2.80-fold increase when this UAE associated with MPR purification (475.42 mg/g dry weight). In vitro cell free antioxidant activity of N. lotus stamen extracts and in cellulo antioxidant investigation using yeast model showed the same trend, indicating that the best antioxidant flavonoid can be found in UAE coupled with MPR purification. Moreover, in the yeast model, the expression of key antioxidant genes such as SIR2 and SOD2 were expressed at the highest level in yeast cells treated with the extract from UAE together with MPR purification. Consequently, it can be seen that the UAE combined with MPR purification can help enhance the flavonoid antioxidant potential of the stamens extract from this medicinal species.

Project description:BackgroundSilk sericin and a few non-protein components isolated from the cocoon layer including two silk proteins in silkworm Bombyx mori has many bioactivities. The dietary sericin possess antinatural oxidation, anticancer, antihyperlipidemic, and antidiabetic activities. The non-protein components surrounding the sericin layer involve in wax, pigments mainly meaning flavonoids, sugars, and other impurities. However, very few investigations have reported the estimation of the total flavonoids derived from the cocoon layer. The flavonoids are commonly present in their glycosylated forms and mostly exist as quercetin glycosides in the sericin layers of silkworm cocoons.ObjectiveThe aim of this study was to find a more accurate method to estimate the level of the total flavonoids in silkworm cocoons.DesignAn efficient procedure of hydrolysis-assisted extraction (HAE) was first established to estimate the level of the total flavonoids through the determination of their aglycones, quercetin, and kaempferol. Then, a comparison was made between traditional colorimetric method and our method. In addition, the antioxidant activities of hydrolysis-assisted extract sample were determined.ResultsThe average contents of quercetin and kaempferol were 1.98 and 0.42 mg/g in Daizo cocoon. Their recoveries were 99.56 and 99.17%. The total sum of quercetin and kaempferol was detected to be 2.40±0.07 mg/g by HAE-HPLC, while the total flavonoids (2.59±0.48 mg/g) estimated by the traditional colorimetric method were only equivalent to 1.28±0.04 mg/g of quercetin. The HAE sample also exhibits that IC50 values of scavenging ability of diphenyl picryl hydrazinyl (DPPH) radical and hydroxyl radical (HO·) are 243.63 µg/mL and 4.89 mg/mL, respectively.ConclusionsThese results show that the HAE-HPLC method is specificity of cocoon and far superior to the colorimetric method. Therefore, this study has profound significance for the comprehensive utilization of silkworm cocoon and also may be applied to the estimation of total flavonoids in other functional foods.

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Purification of total flavonoids from Rhizoma Smilacis Glabrae through cyclodextrin-assisted extraction and resin adsorption.

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Purification of total flavonoids from <i>Rhizoma Smilacis Glabrae</i> through cyclodextrin-assisted extraction and resin adsorption.

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