Ontology highlight
ABSTRACT:
SUBMITTER: Motohashi K
PROVIDER: S-EPMC6478821 | biostudies-other | 2019 Apr
REPOSITORIES: biostudies-other
Scientific reports 20190423 1
An efficient PCR cloning method is indispensable in modern molecular biology, as it can greatly improve the efficiency of DNA cloning processes. Here, I describe the development of three vectors for TA cloning and blunt-end cloning. Specifically, pCRT and pCRZeroT were designed to improve the efficiency of TA cloning. pCRZeroT can also be used with pCRZero to facilitate blunt-end cloning using the ccdB gene. Using pCRZero and pCRZeroT and applying the Golden Gate reaction, I developed a direct P ...[more]