Ontology highlight
ABSTRACT:
SUBMITTER: Oesinghaus L
PROVIDER: S-EPMC6504869 | biostudies-other | 2019 May
REPOSITORIES: biostudies-other
Oesinghaus Lukas L Simmel Friedrich C FC
Nature communications 20190507 1
The CRISPR effector protein Cas12a has been used for a wide variety of applications such as in vivo gene editing and regulation or in vitro DNA sensing. Here, we add programmability to Cas12a-based DNA processing by combining it with strand displacement-based reaction circuits. We first establish a viable strategy for augmenting Cas12a guide RNAs (gRNAs) at their 5' end and then use such 5' extensions to construct strand displacement gRNAs (SD gRNAs) that can be activated by single-stranded RNA ...[more]