Ontology highlight
ABSTRACT:
SUBMITTER: Ahn JK
PROVIDER: S-EPMC6551882 | biostudies-other | 2019
REPOSITORIES: biostudies-other
Ahn Jun Ki JK Kim Hyo Yong HY Lee Chang Yeol CY Park Ki Soo KS Park Hyun Gyu HG
Journal of biological engineering 20190604
We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5'-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a ...[more]