Ontology highlight
ABSTRACT:
SUBMITTER: Kim CS
PROVIDER: S-EPMC6612818 | biostudies-other | 2018 Dec
REPOSITORIES: biostudies-other
Kim Chang Sik CS Mohan Sumitra S Ayub Mahmood M Rothwell Dominic G DG Dive Caroline C Brady Ged G Miller Crispin C
Bioinformatics (Oxford, England) 20190701 14
<h4>Motivation</h4>Circulating-free DNA (cfDNA) profiling by sequencing is an important minimally invasive protocol for monitoring the mutation profile of solid tumours in cancer patients. Since the concentration of available cfDNA is limited, sample library generation relies on multiple rounds of PCR amplification, during which the accumulation of errors results in reduced sensitivity and lower accuracy.<h4>Results</h4>We present PCR Error Correction (PEC), an algorithm to identify and correct ...[more]