Ontology highlight
ABSTRACT:
SUBMITTER: Li B
PROVIDER: S-EPMC6662066 | biostudies-other | 2019 Jul
REPOSITORIES: biostudies-other
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20190502 13
Fluorogenic labeling enables imaging cellular molecules of interest with minimal background. This process is accompanied with the notable increase of the quantum yield of fluorophore, thus minimizing the background signals from unactivated profluorophores. Herein, the development of a highly efficient and bioorthogonal nitroso-based Diels-Alder fluorogenic reaction is presented and its usefulness is validated as effective and controllable in fluorescent probes and live-cell labeling strategies f ...[more]