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Long-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.

ABSTRACT: OBJECTIVE:Although most patients recover from acute Q fever, around 20% develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome that lasts at least 6 months. This study investigated transcriptional profiles of circulating monocytes and circulating cytokines as a subsequent mirror of myeloid cell function, 1 and 6 months after an acute Q fever infection. METHODS:Total RNA of circulating monocytes was collected from 11 acute Q fever patients and 15 healthy controls, matched for age (±5 years) and sex. Samples were collected at a median of 27 days (baseline, interquartile range, 15-35 days) after the infection and again 6 months thereafter. Transcriptome analysis was performed using RNA sequencing. Additionally, concentrations of circulating interleukin (IL)-10, IL-1β, IL-1Ra, and IL-6 were measured in serum. RESULTS:At baseline, acute Q fever patients clearly show a differential transcriptional program compared with healthy controls. This is still the case at follow-up, albeit to a lesser extent. At baseline, a significant difference in levels of circulating IL-10 (P = .0019), IL-1β (P = .0067), IL-1Ra (P = .0008), and IL-6 (P = .0003) was seen. At follow-up, this difference had decreased for IL-10 (P = .0136) and IL-1Ra (P = .0017) and had become nonsignificant for IL-1β (P = .1139) and IL-6 (P = .2792). CONCLUSIONS:We show that an acute Q fever infection has a long-term effect on the transcriptional program of circulating monocytes and, therefore, likely their myeloid progenitor cells, as well as concentrations of circulating IL-10, IL-1β, IL-1Ra, and IL-6.

SUBMITTER: Raijmakers RP

PROVIDER: S-EPMC6667718 | biostudies-other | 2019 Jul

REPOSITORIES: biostudies-other

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Long-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.

Raijmakers Ruud Ph RP Stenos John J Keijmel Stephan P SP Ter Horst Rob R Novakovic Boris B Nguyen Chelsea C Van Der Meer Jos Wm JW Netea Mihai G MG Bleeker-Rovers Chantal P CP Joosten Leo Ab LA Graves Stephen R SR

Open forum infectious diseases 20190701 7

<h4>Objective</h4>Although most patients recover from acute Q fever, around 20% develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome that lasts at least 6 months. This study investigated transcriptional profiles of circulating monocytes and circulating cytokines as a subsequent mirror of myeloid cell function, 1 and 6 months after an acute Q fever infection.<h4>Methods</h4>Total RNA of circulating monocytes was collected from 11 acute Q fever patients and 15 healthy controls, ...[more]

PMID: 31363773

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Long-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.

Publications

Long-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.

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