Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC6703874 | biostudies-other | 2019 Aug
REPOSITORIES: biostudies-other
Zhang Yi Y Minagawa Yoshihiro Y Kizoe Hiroto H Miyazaki Kentaro K Iino Ryota R Ueno Hiroshi H Tabata Kazuhito V KV Shimane Yasuhiro Y Noji Hiroyuki H
Science advances 20190821 8
We report a general strategy based on digital counting principle that enables an efficient acquisition of enzyme mutants with desired activities from just a few clones within a day. We prepared a high-density femtoliter droplet array, consisting of 1 million uniform droplets per 1 cm<sup>2</sup> to carry out high-throughput protein synthesis and screening. Single DNA molecules were randomly distributed into each droplet following a Poisson process to initiate the protein synthesis with coupled c ...[more]