Unknown

Dataset Information

0

Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells.


ABSTRACT: Intercellular communication within the bone marrow niche significantly promotes leukemogenesis and provides protection of leukemic cells from therapy. Secreted factors, intercellular transfer of mitochondria and the receptor-ligand interactions have been shown as mediators of this protection. Here we report that tunneling nanotubes (TNTs)-long, thin membranous structures, which have been identified as a novel mode of intercellular cross-talk-are formed in the presence of stroma and mediate transfer of cellular vesicles from stroma to leukemic cells. Importantly, transmission of vesicles via TNTs from stromal cells increases resistance of leukemic cells to the tyrosine kinase inhibitor, imatinib. Using correlative light-electron microscopy and electron tomography we show that stromal TNTs contain vesicles, provide membrane continuity with the cell bodies and can be open-ended. Moreover, trans-SILAC studies to reveal the non-autonomous proteome showed that specific sets of proteins are transferred together with cellular vesicles from stromal to leukemic cells, with a potential role in survival and adaptation. Altogether, our findings provide evidence for the biological role of the TNT-mediated vesicle exchange between stromal and leukemic cells, implicating the direct vesicle and protein transfer in the stroma-provided protection of leukemic cells.

SUBMITTER: Kolba MD 

PROVIDER: S-EPMC6817823 | biostudies-other | 2019 Oct

REPOSITORIES: biostudies-other

altmetric image

Publications

Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells.

Kolba Marta D MD   Dudka Wioleta W   Zaręba-Kozioł Monika M   Kominek Agata A   Ronchi Paolo P   Turos Laura L   Chroscicki Piotr P   Wlodarczyk Jakub J   Schwab Yannick Y   Klejman Agata A   Cysewski Dominik D   Srpan Katja K   Davis Daniel M DM   Piwocka Katarzyna K  

Cell death & disease 20191028 11


Intercellular communication within the bone marrow niche significantly promotes leukemogenesis and provides protection of leukemic cells from therapy. Secreted factors, intercellular transfer of mitochondria and the receptor-ligand interactions have been shown as mediators of this protection. Here we report that tunneling nanotubes (TNTs)-long, thin membranous structures, which have been identified as a novel mode of intercellular cross-talk-are formed in the presence of stroma and mediate trans  ...[more]

Similar Datasets

| S-EPMC6787037 | biostudies-literature
| S-EPMC5352373 | biostudies-literature
| S-EPMC5683209 | biostudies-literature
2022-02-16 | PXD011013 | Pride
2019-11-11 | PXD013504 | Pride
2024-09-13 | GSE229487 | GEO
| S-EPMC2726068 | biostudies-other
| S-EPMC4713889 | biostudies-literature
| S-EPMC7319929 | biostudies-literature
| S-EPMC5901965 | biostudies-literature