Repurposing an endogenous degradation domain for antibody-mediated disposal of cell-surface proteins

ABSTRACT: The exquisite specificity of antibodies can be harnessed to effect targeted degradation of membrane proteins. Here, we demonstrate targeted protein removal utilising a protein degradation domain derived from the endogenous human protein Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9). Recombinant antibodies genetically fused to this domain drive degradation of membrane proteins that undergo constitutive internalisation and recycling, including the transferrin receptor and the human cytomegalovirus latency-associated protein US28. We term this approach PACTAC (PCSK9-Antibody Clearance-Targeting Chimeras).

SUBMITTER: Ms. Janika Schmitt 

PROVIDER: S-SCDT-10_1038-S44319-024-00063-3 | biostudies-other |

REPOSITORIES: biostudies-other