The functional significance of the RPA- and PCNA-dependent recruitment of Pif1 to DNA

ABSTRACT: Pif1 family helicases are multifunctional proteins conserved in eukaryotes, from yeast to humans. They are important for the genome maintenance in both nuclei and mitochondria, where they have been implicated in Okazaki fragment processing, replication fork progression and termination, telomerase regulation and DNA repair. While the Pif1 helicase activity is readily detectable on naked nucleic acids in vitro, the in vivo functions rely on recruitment to DNA. We identify the single-stranded DNA binding protein complex RPA as the major recruiter of Pif1 in budding yeast, in addition to the previously reported Pif1-PCNA interaction. The two modes of the Pif1 recruitment act independently during telomerase inhibition, as the mutations in the Pif1 motifs disrupting either of the recruitment pat

SUBMITTER: Dr. Svetlana Makovets 

PROVIDER: S-SCDT-10_1038-S44319-024-00114-9 | biostudies-other |

REPOSITORIES: biostudies-other