Centrosome linker diversity and its function in centrosome clustering and mitotic spindle formation

ABSTRACT: The centrosome linker joins the two interphase centrosomes of a cell into one microtubule organizing centre. Despite increasing knowledge on linker components, linker diversity in different cell types and their role in cells with supernumerary centrosomes remained unexplored. Here, we identified Ninein as a C-Nap1-anchored centrosome linker component that provides linker function in RPE1 cells while in HCT116 and U2OS cells Ninein and Rootletin link centrosomes together. In interphase, overamplified centrosomes use the linker for centrosome clustering, where Rootletin gains centrosome linker function in RPE1 cells. Surprisingly, in cells with centrosome overamplification, C-Nap1 loss prolongs metaphase through persistent activation of the spindle assembly checkpoint indicated by BUB1 and M

SUBMITTER: Prof. Elmar Schiebel 

PROVIDER: S-SCDT-10_15252-EMBJ_2021109738 | biostudies-other |

REPOSITORIES: biostudies-other