An actin remodeling role for Arabidopsis processing bodies revealed by their proximity interactome

ABSTRACT: Cellular condensates can comprise membrane-less ribonucleoprotein assemblies with liquid-like properties. These cellular condensates influence various biological outcomes, but their liquidity hampers their isolation and characterization. Here, we investigated the composition of the condensates known as processing bodies (PBs) in the model plant Arabidopsis through a proximity-biotinylation pipeline. Using in situ protein-protein interaction approaches, genetics and high-resolution dynamic imaging, we show that PBs comprise networks that interface with membranes. Surprisingly, the conserved component of PBs, DECAPPING PROTEIN 1 (DCP1), can interface with unique plasma membrane subdomains that include cell edges and vertices. We characterized these plasma membrane interfaces and discovered a

SUBMITTER: Chen Liu 

PROVIDER: S-SCDT-10_15252-EMBJ_2022111885 | biostudies-other |

REPOSITORIES: biostudies-other