Acetylation Coordinates the Crosstalk between Carbon Metabolism and Ammonium Assimilation in Salmonella enterica

ABSTRACT: Enteric bacteria use up to 15% of their cellular energy for ammonium assimilation via glutamine synthetase (GS)/glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) in response to varying ammonium availability. However, the sensory mechanisms for effective and appropriate coordination between carbon metabolism and ammonium assimilation have not been fully elucidated. Here, we report that in Salmonella enterica, carbon metabolism coordinates the activities of GS/GDH via functionally reversible protein lysine acetylation. Glucose promotes Pat acetyltransferase-mediated acetylation and activation of adenylylated GS. Simultaneously, glucose induces GDH acetylation to inactivate the enzyme by impeding its catalytic centre, which is reversed upon GDH deacetylation by deacetylase CobB. Mo

SUBMITTER: Dr. Yunwei Sun 

PROVIDER: S-SCDT-10_15252-EMBJ_2022112333 | biostudies-other |

REPOSITORIES: biostudies-other