The chromatin factor ROW cooperates with BEAF-32 in regulating long-range inducible genes

ABSTRACT: Insulator proteins located at the boundaries of topological associated domains (TAD) are involved in higher-order chromatin organization and transcription regulation. However, it is still not clear how long-range contacts contribute to transcriptional regulation. Here we show that Relative-of-WOC (ROW) is essential for the long-range transcription regulation mediated by the Boundary Element-Associated Factor of 32kD (BEAF-32). We find that ROW physically interacts with heterochromatin proteins (HP1b and HP1c) and the insulator protein BEAF-32. These proteins interact at TAD boundaries where ROW, through its AT-hook motifs, binds AT-rich sequences flanked by BEAF-32 binding sites and motifs. Knockdown of row downregulates genes that are long-range targets of BEAF-32 and bound indirectly by

SUBMITTER: Ms. Neta Herman 

PROVIDER: S-SCDT-10_15252-EMBR_202254720 | biostudies-other |

REPOSITORIES: biostudies-other