Jag2b-Notch3/1b-mediated neuron-to-glia crosstalk controls retinal gliogenesis

ABSTRACT: In the developing central nervous systems (CNS), neural progenitor cells generate neurons and glia in a sequential order. However, the influence of neurons on glia generation remains elusive. Here we report that photoreceptor cell-derived Jag2b is required for Notch-dependent Müller glia (MG) generation in the developing zebrafish retina. In jab2b-/- mutants, differentiating MGs are re-specified into lineage-related bipolar neuron fate at the expense of mature MG. Single-cell transcriptome analysis and knock-in animals reveal that jab2b is specifically expressed in crx+-photoreceptor cells during MG generation. Crx promoter-driven jag2b, but not other Notch ligands, is sufficient to rescue the loss of MGs observed in jag2b-/- mutants. Furthermore, we observe a severe and moderate decrease

SUBMITTER: Mr. Mengmeng Jin 

PROVIDER: S-SCDT-10_15252-EMBR_202254922 | biostudies-other |

REPOSITORIES: biostudies-other