Error-free DNA damage tolerance pathway is facilitated by the Irc5 translocase through cohesin

ABSTRACT: DNA damage tolerance (DDT) mechanisms facilitate replication resumption and completion when DNA replication is blocked by bulky DNA lesions. In budding yeast, template switching (TS) via the Rad18/Rad5 pathway is a favored DDT pathway that involves usage of the sister chromatid as a template to bypass DNA lesions in an error-free recombination-like process. Here, we establish that the Snf2 family translocase Irc5 is a novel factor that promotes TS and averts single-stranded DNA persistence during replication. We demonstrate that during replication stress, Irc5 enables replication progression by assisting enrichment of cohesin complexes, recruited in an Scc2/Scc4-dependent fashion, near blocked replication forks. This allows efficient formation of sister chromatid junctions that are crucial

SUBMITTER: Dr. Ireneusz, Bernard Litwin 

PROVIDER: S-SCDT-EMBOJ-2017-98732 | biostudies-other |

REPOSITORIES: biostudies-other