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Loss of T-bet confers survival advantage to influenza-bacterial superinfection


ABSTRACT: The transcription factor, T-bet, regulates type 1 inflammatory responses against a range of infections. Here, we demonstrate a previously unaddressed role of T-bet, to influenza virus and bacterial superinfection. Interestingly, we found that T-bet deficiency did not adversely affect the efficacy of viral clearance or recovery compared to wild-type hosts. Instead, increased infiltration of neutrophils and production of Th17 cytokines (IL17 and IL-22), in lungs of influenza virus infected T-bet-/- mice was correlated with survival advantage against subsequent infection by Streptococcus pneumoniae. Neutralization of IL-17, but not of IL-22 in T-bet-/- mice increased pulmonary bacterial load, concomitant with decreased neutrophil infiltration and reduced survival of T-bet-/- mice. IL-17 production by CD8+, CD4+ and γδ T cell types were identified to contribute to this protection against bacterial superinfection. We further showed that neutrophil depletion in T-bet-/- lungs increased pulmonary bacterial burden. These results thus indicate that despite the loss of T-bet, immune defences required for influenza viral clearance are fully functional, which in turn enhances protective type 17 immune responses against lethal bacterial superinfections.

SUBMITTER: Prof. Jeak, Ling Ding 

PROVIDER: S-SCDT-EMBOJ-2018-99176 | biostudies-other |

REPOSITORIES: biostudies-other

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