Proteasomal degradation within endocytic organelles mediates antigen cross-presentation
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ABSTRACT: During MHC-I-restricted antigen processing, peptides generated by cytosolic proteasomes are translocated by the Transporter associated with Antigen Processing (TAP) into the endoplasmic reticulum, where they bind to newly synthesized MHC-I molecules. Dendritic cells and other cell types can also generate MHC-I complexes with peptides derived from internalized proteins, a process called cross-presentation. Here we show that active proteasomes within cross-presenting cell phagosomes can generate these peptides. Active proteasomes are detectable within endocytic compartments in mouse bone marrow-derived dendritic cells. In TAP-deficient mouse dendritic cells cross-presentation is enhanced by the introduction of human ?2-microglobulin, which increases surface expression of MHC-I and suggests a role for recycling MHC-I molecules. In addition, surface MHC-I can be reduced by proteasome inhibition and stabilized by MHC-I-restricted peptides. This is consistent with constitutive proteasome-dependent but TAP-independent peptide loading in the endocytic pathway. Rab-GTPase mutants that restrain phagosome maturation increase proteasome recruitment and enhance TAP-independent cross-presentation. Thus, phagosomal/endosomal binding of peptides locally generated by proteasomes allows cross-presentation to generate MHC-I- peptide complexes identical to those produced by conventional antigen processing.
SUBMITTER: Dr. Peter Cresswell
PROVIDER: S-SCDT-EMBOJ-2018-99266 | biostudies-other |
REPOSITORIES: biostudies-other
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