The RNA Polymerase II subunit RPB-9 recruits the Integrator complex to terminate C. elegans piRNA transcription

ABSTRACT: PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate germ cell development and maintain germline integrity in many animals. Mature piRNAs engage Piwi Argonaute proteins to silence complementary transcripts, including transposable elements and endogenous genes. piRNA biogenesis mechanisms are diverse and remain poorly understood. Here, we identify the RNA Polymerase II (RNA Pol II) core subunit RPB-9 as required for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We show that rpb-9 initiates heritable piRNA-mediated gene silencing at two DNA transposon families and at a subset of somatic genes in the germline. We provide genetic and biochemical evidence that RPB-9 is required for piRNA biogenesis by recruiting the Integrator complex at piRNA genes, hen

SUBMITTER: Dr. Ahmet C. Berkyurek 

PROVIDER: S-SCDT-EMBOJ-2020-105565 | biostudies-other |

REPOSITORIES: biostudies-other