Controlled X-chromosome activation dynamics defines meiotic potential of female mouse in vitro germ cells

ABSTRACT: The mammalian germline is characterised by extensive epigenetic reprogramming during its development into functional eggs and sperm. Specifically, the epigenome requires resetting before parental marks can be established and transmitted to the next generation. In the female germline, X-chromosome inactivation and reactivation are among the most prominent epigenetic reprogramming events, yet very little is known about their kinetics and biological function. Here, we investigate X-inactivation and reactivation dynamics using a tailor-made in vitro system of primordial germ cell-like cell (PGCLC) differentiation from mouse embryonic stem cells. We find that X-inactivation in PGCLCs in vitro and in germ cell-competent epiblast cells in vivo is moderate compared to somatic cells, and frequently

SUBMITTER: Dr. Jacqueline Serverino 

PROVIDER: S-SCDT-EMBOJ-2021-109457 | biostudies-other |

REPOSITORIES: biostudies-other