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Controlled X-chromosome activation dynamics defines meiotic potential of female mouse in vitro germ cells

ABSTRACT: The mammalian germline is characterised by extensive epigenetic reprogramming during its development into functional eggs and sperm. Specifically, the epigenome requires resetting before parental marks can be established and transmitted to the next generation. In the female germline, X-chromosome inactivation and reactivation are among the most prominent epigenetic reprogramming events, yet very little is known about their kinetics and biological function. Here, we investigate X-inactivation and reactivation dynamics using a tailor-made in vitro system of primordial germ cell-like cell (PGCLC) differentiation from mouse embryonic stem cells. We find that X-inactivation in PGCLCs in vitro and in germ cell-competent epiblast cells in vivo is moderate compared to somatic cells, and frequently characterised by escaping genes. X-inactivation is followed by step-wise X-reactivation, which is mostly completed during meiotic prophase I. Furthermore, we find that PGCLCs which fail to undergo X-inactivation or reactivate too rapidly display impaired meiotic potential. Thus, our data reveals fine-tuned X-chromosome remodelling as a critical feature of female germ cell development towards meiosis and oogenesis.

SUBMITTER: Dr. Jacqueline Serverino

PROVIDER: S-SCDT-EMBOJ-2021-109457 | biostudies-other |

REPOSITORIES: biostudies-other

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