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THE DUAL ROLE OF THE CENTROSOME IN ORGANIZING THE MICROTUBULE NETWORK IN INTERPHASE

ABSTRACT: Here we address the regulation of microtubule nucleation during interphase by genetically ablating one, or two, of three major mammalian ?-TuRC-binding factors namely pericentrin, CDK5Rap2 and AKAP450. Unexpectedly, we find that while all of them participate in microtubule nucleation at the Golgi apparatus, they only modestly contribute at the centrosome where CEP192 has a more predominant function. We also show that inhibiting microtubule nucleation at the Golgi does not affect centrosomal activity whereas manipulating the number of centrosomes with centrinone modifies microtubule nucleation activity of the Golgi apparatus. In centrosome-free cells, inhibition of Golgi-based microtubule nucleation triggers pericentrin-dependent formation of cytoplasmic nucleating structures. Further depletion of pericentrin under these conditions leads to the generation of individual microtubules in a ?-tubulin dependent manner. In all cases, a conspicuous MT network forms. Strikingly, centrosome loss increases microtubule number independently of where they were growing from. Our results lead to an unexpected view of the interphase centrosome that would control microtubule network organization not only by nucleating microtubules, but also by modulating the activity of alternative microtubule-organizing centers.

SUBMITTER: Dr. Maria, P Gavilan

PROVIDER: S-SCDT-EMBOR-2018-45942-T | biostudies-other |

REPOSITORIES: biostudies-other

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