Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1

ABSTRACT: Snail is a dedicated transcriptional repressor and acts as a master inducer of EMT and metastasis, yet the underlying signaling cascades triggered by Snail still remain elusive. Here we report that Snail promotes colorectal cancer (CRC) migration by preventing non-coding RNA LOC113230-mediated degradation of arginosuccinate synthase 1 (ASS1). LOC113230 is a novel Snail target gene, and Snail binds to the functional E-boxes within its proximal promoter to repress its expression in response to TGF-? induction. Ectopic expression of LOC113230 potently suppresses CRC cell growth, migration and lung metastasis in xenograft experiments. Mechanistically, LOC113230 acts as a scaffold to facilitate recruiting LRPPRC and the TRAF2 E3 ubiquitin ligase to ASS1, resulting in enhanced ubiquitination and

SUBMITTER: Prof. Hao Jia 

PROVIDER: S-SCDT-EMBOR-2020-51780-T | biostudies-other |

REPOSITORIES: biostudies-other