Male infertility associated Ccdc108 Regulates Multiciliogenesis via the Intraflagellar Transport Machinery

ABSTRACT: Motile cilia on the cell surface generate movement and directional fluid flow that is crucial for various biological processes. Dysfunction of these cilia causes human diseases such as sinopulmonary disease and infertility. Here, we show that Ccdc108, a protein linked to male infertility, has an evolutionarily conserved requirement in motile multiciliation. Using Xenopus laevis embryos, Ccdc108 is shown to be required for the migration and docking of basal bodies to the apical membrane in epidermal multiciliated cells (MCCs). We demonstrate that Ccdc108 interacts with the IFT-B complex, and the ciliation requirement for Ift74 overlaps with Ccdc108 in MCCs. Both Ccdc108 and IFT-B proteins localize to migrating centrioles, basal bodies, and cilia in MCCs. Importantly, Ccdc108 governs the cen

SUBMITTER: Dr. Huijie Zhao 

PROVIDER: S-SCDT-EMBOR-2021-52775-T | biostudies-other |

REPOSITORIES: biostudies-other