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Transcriptomic characterisation of haematopoietic stem and progenitor cells from human adult bone marrow, spleen and peripheral blood

ABSTRACT: Haematopoietic stem and progenitor cells (HSPCs), the precursors of all blood cells, reside predominantly in the bone marrow (BM). Recent evidence suggests that other anatomical sites may contribute significantly to blood production, but the cellular, molecular and functional composition of extramedullary HSPC pools remains unexplored. Here, we comprehensively characterized the single-cell composition of the adult human HSPC pool within matched BM, spleen and peripheral blood (PB) from four healthy organ donors. 10x scRNA-seq of >65,000 HSPCs from all three sites defined transcriptionally distinct cell clusters that correspond to precursors of all haematopoietic lineages but also stem cells (HSCs). We find that, contrary to actively proliferating BM HSPCs, extramedullary tissues are enriched for low-to-non proliferating early progenitors, indicative of no significant haematopoiesis in these tissues at steady-state. We further describe a shared transcriptional signature of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) at extramedullary sites and the increased abundance of lineage-primed subsets, that distinguishes them from BM HSC/MPPs. This dataset is part of the Human Cell Atlas.

ORGANISM(S): Homo sapiens

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PROVIDER: S-SUBS4 | biostudies-other |

SECONDARY ACCESSION(S): PRJEB36885

REPOSITORIES: biostudies-other

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