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Tissue Specimens from Patients with Melanoma for Genetic Analysis

ABSTRACT:

The purpose of this study was to obtain tissue specimens derived from patients with melanoma to generate research tools to advance our understanding of the genetics, pathogenesis, and therapeutics of melanoma. Briefly, tissue was obtained from metastatic lesions and used to generate clonal primary cell lines from melanoma cells and fibroblasts from the tumor microenvironment. RNA was extracted from low passage cell lines using Trizol reagent. cDNA libraries were prepared using the TruSeq mRNA sample preparation kit, v2 (Illumina) and sequenced on the HiSeq 2000 platform (Illumina). The submitted files are bam files that contain both unaligned and aligned reads (human genome, build hg19).

PROVIDER: phs001115 | dbGaP |

SECONDARY ACCESSION(S): PRJNA320662PRJNA320661

REPOSITORIES: dbGaP

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	GapExchange_phs001115.v1.p1.xml	Xml
	dbGaPEx2.1.5.xsd	Other
	phs001115.v1-Documents.zip	Other
	Study_Report.phs001115.MelanomaGeneticAnalysis.v1.p1.MULTI.pdf	Pdf
	manifest_phs001115.MelanomaGeneticAnalysis.v1.p1.c1.GRU.pdf	Pdf

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