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High-Throughput Determination of the Antigen Specificities of T cell Receptors in Single Cells

ABSTRACT:

High-throughput linking of T cell receptor (TCR) sequences to their binding antigens is vital for immune profiling, yet challenging. We present Tetramer associated TCR Sequencing (TetTCR-Seq) to address this challenge. Binding is determined using a library of DNA-barcoded antigen tetramers that are rapidly and inexpensively generated using an in vitro transcription/translation platform. We included CMV+ donors (CMV seropositive donors who are infected with Cytomegalovirus) to screen for CMV specific TCRs.

PROVIDER: phs001678 | dbGaP |

REPOSITORIES: dbGaP

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			Action	DRS
	GapExchange_phs001678.v1.p1.xml	Xml
	dbGaPEx2.1.5.xsd	Other
	Study_Report.phs001678.scTCellReceptors.v1.p1.MULTI.pdf	Pdf
	manifest_phs001678.scTCellReceptors.v1.p1.c1.HMB-IRB-NPU.pdf	Pdf
	datadict_v2.xsl	Other

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