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MNGS of Pulmonary Fluid in Immunocompromised Children

ABSTRACT:

Despite improved diagnostics, pulmonary pathogens in immunocompromised children frequently evade detection, leading to significant mortality. In this study, we performed RNA and DNA-based metagenomic next generation sequencing (mNGS) on 41 lower respiratory samples collected from 34 children. We identified a rich cross-domain pulmonary microbiome containing bacteria, fungi, RNA viruses, and DNA viruses in each patient. Potentially pathogenic bacteria were ubiquitous among samples but could be distinguished as possible causes of disease by parsing for outlier organisms. Potential pathogens were detected in half of samples previously negative by clinical diagnostics. Ongoing investigation is needed to determine the pathogenic significance of outlier microbes in the lungs of immunocompromised children with pulmonary disease. Metatranscriptomic (RNA) sequencing libraries are reported in the manuscript and are included for this release.

PROVIDER: phs001684 | dbGaP |

REPOSITORIES: dbGaP

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			Action	DRS
	GapExchange_phs001684.v1.p1.xml	Xml
	dbGaPEx2.1.5.xsd	Other
	Study_Report.phs001684.PulmonaryFluidImmunocompromised.v1.p1.MULTI.pdf	Pdf
	manifest_phs001684.PulmonaryFluidImmunocompromised.v1.p1.c1.HMB-IRB-COL-MDS.pdf	Pdf
	datadict_v2.xsl	Other

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