Optimisation and Performance of Reduced Preparation CT Colography
Ontology highlight
ABSTRACT:
DISEASE(S): Patients With Potential Colorectal Neoplasia,Neoplasms
PROVIDER: 2020435 | ecrin-mdr-crc |
REPOSITORIES: ECRIN MDR
Similar Datasets
Project description:To establish the optimum barium-based reduced-laxative tagging regimen prior to CT colonography (CTC). Ninety-five subjects underwent reduced-laxative (13 g senna/18 g magnesium citrate) CTC prior to same-day colonoscopy and were randomised to one of four tagging regimens using 20 ml 40%w/v barium sulphate: regimen A: four doses, B: three doses, C: three doses plus 220 ml 2.1% barium sulphate, or D: three doses plus 15 ml diatriazoate megluamine. Patient experience was assessed immediately after CTC and 1 week later. Two radiologists graded residual stool (1: none/scattered to 4: >50% circumference) and tagging efficacy for stool (1: untagged to 5: 100% tagged) and fluid (1: untagged, 2: layered, 3: tagged), noting the HU of tagged fluid. Preparation was good (76-94% segments graded 1), although best for regimen D (P = 0.02). Across all regimens, stool tagging quality was high (mean 3.7-4.5) and not significantly different among regimens. The HU of layered tagged fluid was higher for regimens C/D than A/B (P = 0.002). Detection of cancer (n = 2), polyps > or =6 mm (n = 21), and < or =5 mm (n = 72) was 100, 81 and 32% respectively, with only four false positives > or =6 mm. Reduced preparation was tolerated better than full endoscopic preparation by 61%. Reduced-laxative CTC with three doses of 20 ml 40% barium sulphate is as effective as more complex regimens, retaining adequate diagnostic accuracy.
| S-EPMC2220024 | biostudies-other
Project description:The study will compare patient’s experience between those taking a standard bowel cleansing regimen with minimal laxative tagging regimen of senna and gastrofin. Additionally comparing any possible reduction in diagnostic accuracy that may ensue from an increased quantity of retained faecal residue.
| 2039044 | ecrin-mdr-crc
Project description:Dhokla, a popular indigenous savoury dish of India, is prepared by soaking bengalgram dal and rice, grinding separately, mixing the batters, and spontaneously fermenting and steaming of mixed batter. Central composite rotatable response surface designs for soaking, fermentation and steaming at five-level combinations were used for optimising preparation of dhokla to achieve reduced contents of antinutrients. Optimum soaking of bengalgram dal (dal-water ratio of 1:5 w/w, pH 7.0, 23 °C, 20 h) and rice (rice-water ratio of 1:5 w/w, pH 5.6, 16 °C, 18 h) resulted in reduced levels of all the antinutrients, except total biogenic amines in rice. Fermentation of dal-rice (3:1 v/v) mixed batter under optimum condition (added NaCl of 8 g/kg, 32 °C, 18 h) further reduced their levels, but total biogenic amines content was enhanced. However, optimum steaming of dal-rice mixed fermented batter for 20 min was effective in reducing all the tested antinutrients. In dhokla, the content of tannins, phytic acid and total biogenic amines reduced by 100, 94 and 20%, respectively; trypsin inhibitor and haemagglutinating activities reduced by 92 and 100%, respectively, over raw ingredients. The optimally prepared product (dhokla) ranked "excellent" in terms of overall sensory quality.
| S-EPMC5976588 | biostudies-literature
Project description:The rete ovarii (RO) is an epithelial structure that arises during fetal development in close proximity to the ovary, and persists throughout adulthood in mammals. However, the functional significance of the RO remains elusive and it has been absent from recent discussions of female reproductive anatomy. The RO comprises three distinct regions: the intraovarian rete (IOR) within the ovary, the extraovarian rete (EOR) in the periovarian tissue, and the connecting rete (CR) linking the EOR and IOR. We hypothesize that the RO plays a pivotal role in maintaining ovarian homeostasis and responding to physiological changes. To uncover the nature and function of RO cells, we conducted transcriptome analysis, encompassing bulk, single-cell, and nucleus-level sequencing of both fetal and adult RO tissues using the Pax8-rtTA; Tre-H2B-GFP mouse line, where all RO regions express nuclear GFP. This study presents three datasets, which highlight RO-specific gene expression signatures and reveal differences in gene expression across the three RO regions during development and in adulthood. The integration and rigorous validation of these datasets will advance our understanding of the RO's roles in ovarian development, female maturation, and adult female fertility.
2023-11-07 | GSE244849 | GEO
Project description:RNA binding proteins (RBPs) are key regulators of normal and pathological gene expression. Small molecules targeting RBP-RNA interactions are a rapidly emerging class of therapeutics. Ro-08-2750 (Ro) was previously identified as a competitive inhibitor of Musashi-RNA interactions. Here we show Ro potently inhibits steroidogenesis and viability independent of Musashi-2 in multiple cell lines. We identified Ro-interacting proteins using an unbiased proteome-wide approach. Other RBPs were a prominent class of Ro-interacting proteins and leveraging large-scale ENCODE data we found a subset of these phenocopy Ro inhibition. We provide a general framework for validating the specificity and identifying targets of RBP inhibitors in a cellular context.
2022-09-14 | GSE213218 | GEO
Project description:Introduction:The Slovenian Resolution on the National Healthcare Plan notes that the country's medical laboratory activities are fragmented, which may result in cost-inefficiency and a reduction in the quality of the services provided. Defining the efficiency of laboratory service providers can therefore help us to pursue the objectives of the Resolution, i.e. to consolidate and integrate laboratory activities. Methods:Using the DEA method, we conducted an analysis of the efficiency of 20 biomedical laboratories in Slovenia, and made a comparison with a "virtual" laboratory, i.e. a merger of laboratories within a selected organisational unit. By testing different DEA models, we sought to determine whether the use of different input variables caused significant differences in the laboratories' efficiency scores. Results:The research results show that inefficiency resulting from the size of the units is 1.5 times greater than process inefficiency. Using a non-parametric Wilcoxon Signed Rank test, we determined, at a risk level of 0.05, that there was no difference between the efficiency results when using two different technical efficiency DEA models. When evaluating the virtually merged laboratory, we determined that, under all three models, the virtual laboratory achieved 100% VRS efficiency. However, when the CRS methodology was used, the laboratory showed a certain degree of scale inefficiency. Conclusions:When evaluating merger of medical laboratories we note that the DEA method is methodologically suitable for evaluating the effects of health policy implementation, and is an appropriate tool for identifying where the field of laboratory medicine might be further developed and improved.
| S-EPMC7478087 | biostudies-literature
Project description:Colonoscopy is the technique of choice for the evaluation of the mucosa of the colon. To be able to do the procedure in optimal conditions, it needs to be clean. Therefore, it is necessary to carry out a preparation as safe and tolerable as possible. This preparation generally consists in a low residue diet the days before the colonoscopy and in the intake of a laxative solution. In previous studies, it has been shown that the low residue diet does not play a relevant role as it was considered before. Recent studies demonstrated that reducing the days of low residue diet does not worsen the cleansing and improves the patient experience. The results of this study are required for determining the role of diet restrictive diet in colon cleansing.
| 2388756 | ecrin-mdr-crc
Project description:Hybrid performance in maize predicted with combinations of omics data
| PRJNA416346 | ENA
Project description:Interventions: ultra low dose CT colonography
Primary outcome(s): Per-patient performance of CT ultra low dose colonography in the detection of neoplasms, with colonoscopy results as the reference standard.
Study Design: Single arm Non-randomized
| 2657401 | ecrin-mdr-crc
Project description:Mathematical modelling of signalling pathways aids experimental investigation in system and synthetic biology. Ever increasing data availability prompts the development of large dynamic models with numerous parameters. In this paper, we investigate how the number of unknown parameters affects the convergence of three frequently used optimisation algorithms and four objective functions. We compare objective functions that use data-driven normalisation of the simulations with those that use scaling factors. The data-driven normalisation of the simulation approach implies that simulations are normalised in the same way as the data, making both directly comparable. The scaling factor approach, which is commonly used for parameter estimation in dynamic systems, introduces scaling factors that multiply the simulations to convert them to the scale of the data. Here we show that the scaling factor approach increases, compared to data-driven normalisation of the simulations, the degree of practical non-identifiability, defined as the number of directions in the parameter space, along which parameters are not identifiable. Further, the results indicate that data-driven normalisation of the simulations greatly improve the speed of convergence of all tested algorithms when the overall number of unknown parameters is relatively large (74 parameters in our test problems). Data-driven normalisation of the simulations also markedly improve the performance of the non-gradient-based algorithm tested even when the number of unknown parameters is relatively small (10 parameters in our test problems). As the models and the unknown parameters increase in size, the data-driven normalisation of the simulation approach can be the preferred option, because it does not aggravate non-identifiability and allows for obtaining parameter estimates in a reasonable amount of time.
| S-EPMC5548920 | biostudies-literature