Colorectal Polypectomy Upon Insertion And Withdrawal Or Upon Withdrawal Only?
Ontology highlight
ABSTRACT:
DISEASE(S): Colorectal Cancer,Polyps,Colorectal Polyps
PROVIDER: 2126063 | ecrin-mdr-crc |
REPOSITORIES: ECRIN MDR
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Project description:The preferential localization of some neoplasms, such as serrated polyps, in specific areas of the intestine suggests that non-genetic factors may be important for their development. To test this hypothesis, we took advantage of transgenic mice that expressed HB-EGF throughout the intestine, but develop serrated polyps only in the cecum. Here we show that a host-specific microbiome was associated with serrated polyps, and that alterations of the microbiota induced by antibiotic treatment or by embryo-transfer rederivation markedly inhibited the formation of serrated polyps in the cecum. Mechanistically, development of serrated polyps was associated with a local decrease in epithelial barrier-function, bacterial invasion, production of antimicrobials, and increased expression of several inflammatory factors such as IL-17, Cxcl2, Tnf-α, and IL-1. Increased number of neutrophils were found within the serrated polyps, and their depletion significantly reduced polyp growth. Together these results indicate that non-genetic factors contribute to the development of serrated polyps and suggest that the development of these intestinal neoplasms in the cecum is driven by the interplay between genetic changes in the host, an inflammatory response, and a host-specific microbiota.
2014-02-12 | GSE47735 | GEO
Project description:The preferential localization of some neoplasms, such as serrated polyps, in specific areas of the intestine suggests that non-genetic factors may be important for their development. To test this hypothesis, we took advantage of transgenic mice that expressed HB-EGF throughout the intestine, but develop serrated polyps only in the cecum. Here we show that a host-specific microbiome was associated with serrated polyps, and that alterations of the microbiota induced by antibiotic treatment or by embryo-transfer rederivation markedly inhibited the formation of serrated polyps in the cecum. Mechanistically, development of serrated polyps was associated with a local decrease in epithelial barrier-function, bacterial invasion, production of antimicrobials, and increased expression of several inflammatory factors such as IL-17, Cxcl2, Tnf-α, and IL-1. Increased number of neutrophils were found within the serrated polyps, and their depletion significantly reduced polyp growth. Together these results indicate that non-genetic factors contribute to the development of serrated polyps and suggest that the development of these intestinal neoplasms in the cecum is driven by the interplay between genetic changes in the host, an inflammatory response, and a host-specific microbiota.
2014-02-12 | GSE47734 | GEO
Project description:The preferential localization of some neoplasms, such as serrated polyps, in specific areas of the intestine suggests that non-genetic factors may be important for their development. To test this hypothesis, we took advantage of transgenic mice that expressed HB-EGF throughout the intestine, but develop serrated polyps only in the cecum. Here we show that a host-specific microbiome was associated with serrated polyps, and that alterations of the microbiota induced by antibiotic treatment or by embryo-transfer rederivation markedly inhibited the formation of serrated polyps in the cecum. Mechanistically, development of serrated polyps was associated with a local decrease in epithelial barrier-function, bacterial invasion, production of antimicrobials, and increased expression of several inflammatory factors such as IL-17, Cxcl2, Tnf-M-NM-1, and IL-1. Increased number of neutrophils were found within the serrated polyps, and their depletion significantly reduced polyp growth. Together these results indicate that non-genetic factors contribute to the development of serrated polyps and suggest that the development of these intestinal neoplasms in the cecum is driven by the interplay between genetic changes in the host, an inflammatory response, and a host-specific microbiota. Paired design; Surrounding and SP samples were obtained from the same mouse (n=3; mouse 1, 2, 3)
2014-02-12 | E-GEOD-47734 | biostudies-arrayexpress
Project description:The preferential localization of some neoplasms, such as serrated polyps, in specific areas of the intestine suggests that non-genetic factors may be important for their development. To test this hypothesis, we took advantage of transgenic mice that expressed HB-EGF throughout the intestine, but develop serrated polyps only in the cecum. Here we show that a host-specific microbiome was associated with serrated polyps, and that alterations of the microbiota induced by antibiotic treatment or by embryo-transfer rederivation markedly inhibited the formation of serrated polyps in the cecum. Mechanistically, development of serrated polyps was associated with a local decrease in epithelial barrier-function, bacterial invasion, production of antimicrobials, and increased expression of several inflammatory factors such as IL-17, Cxcl2, Tnf-α, and IL-1. Increased number of neutrophils were found within the serrated polyps, and their depletion significantly reduced polyp growth. Together these results indicate that non-genetic factors contribute to the development of serrated polyps and suggest that the development of these intestinal neoplasms in the cecum is driven by the interplay between genetic changes in the host, an inflammatory response, and a host-specific microbiota. SUMMARY: Serrated polyps (SP) are a heterogeneous group of neoplasms found in particular areas of the gut. To define the factors contributing to their specific localization, we analyzed a strain of transgenic mice that carry a genetic alteration throughout the intestinal epithelium, but only develop SP in the cecum. Transcriptome and immunostaining analyses showed increased expression of antimicrobial genes, inflammatory factors, and the presence of bacteria within SP. Alteration of the cecal microbiota by antibiotic treatment or by embryo-transfer rederivation dramatically reduced SP incidence. Microbiome analysis implicated a limited set of bacteria in the development of SP. Together, these results point to a crucial role for the microbiota in the localized development of SP in a genetically susceptible host. We obtained serrated polyp (SP) and surrounding normal (NM) tissue from the ceca of three affected mice (paired design) and assessed expression differences by RNA-Seq.
2014-02-12 | E-GEOD-47735 | biostudies-arrayexpress
Project description:Interventions: Drug : 1. WLE-Fluorescence Group
?Insert colonoscopy with a transparent distal attachment under white light endoscopy (WLE).
?Intravenous administration of fluorescein sodium 1 ampule (10%, 5 mL) after cecal intubation
?WLE examination from cecum to hepatic flexure and record lesions detected.
?Insert colonoscopy under WLE up to cecum and then fluorescence endoscopic examination from cecum to hepatic flexure. Record and remove lesions detected.
?WLE examination from transverse colon to splenic flexure and record lesions detected.
?Insert colonoscopy under WLE up to hepatic flexure and then fluorescence endoscopic examination from transverse colon to splenic flexure. Record and remove lesions detected.
?WLE examination from descending colon to rectum and record lesions detected.
?Insert colonoscopy under WLE up to descending colon and then fluorescence endoscopic examination from descending colon to rectum. Record and remove lesions detected.
2. Fluorescence-WLE Group
?Insert colonoscopy with a transparent distal attachment under white light endoscopy (WLE).
?Intravenous administration of fluorescein sodium 1 ampule (10%, 5 mL) after cecal intubation
?Fluorescence endoscopic examination from cecum to hepatic flexure and record the lesions detected
?Insert colonoscopy under WLE up to cecum and then WLE examination from cecum to hepatic flexure. Record and remove the lesions detected.
?Fluorescence endoscopic examination from transverse colon to splenic flexure and record the lesions detected
?Insert colonoscopy under WLE up to hepatic flexure and then WLE examination from transverse colon to splenic flexure. Record and remove lesions detected.
?Fluorescence endoscopic examination from descending colon to r
Primary outcome(s): Number of fluorescence positive polyps
Primary Purpose : Diagnosis, Intervention Model : Cross-over, Blinding/Masking : Open, Allocation : RCT
| 2513165 | ecrin-mdr-crc
Project description:This study aims to investigate the comparative effectiveness between polyp removal with or without electrical current. The investigators want to include 600 polyps in the trial.
It is known that polyps have the potential to develop to cancer if left in situ, but the investigators do not know the best way to remove them completely.
| 2193079 | ecrin-mdr-crc
Project description:The strategy of a novel way to enhance bacterial motility
| PRJNA693394 | ENA
Project description:The strategy of a novel way to enhance bacterial motility
| PRJNA689933 | ENA
Project description:A more in-depth exploration of gut functional aspects may be interesting in order to provide hints for action (e.g. dietary strategies) to favor gut balance maintenance (Sinha et al., 2017), given the important role of the intestine in development of possible metabolic diseases. A careful survey on the differential gene expression may help to scouting new interesting functions and identify potential markers for testing various experimental factors. The transcriptomes of the jejunum and cecum mucosae of 19 broiler chickens were compared. At slaughter age (day 42), on 38 birds, selected with a homogeneous body weight, jejunum and cecum mucosae were collected by gently scraping after tissues rinsing in PSB to remove residues of digesta, and immediately frozen in liquid nitrogen and then stored at -80°C. From both tissues, total RNA was extracted using GeneJET RNA Purification Kit (Thermo Scientific)
2019-06-24 | GSE124066 | GEO