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DAT_CancerMethodsPaper_DNAAmounts - samples

ABSTRACT: "Usage of small amounts of DNA for Illumina sequencing"

PROVIDER: EGAD00001000034 | EGA |

REPOSITORIES: EGA

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Publications

Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity.

Kerick Martin M Isau Melanie M Timmermann Bernd B Sültmann Holger H Herwig Ralf R Krobitsch Sylvia S Schaefer Georg G Verdorfer Irmgard I Bartsch Georg G Klocker Helmut H Lehrach Hans H Schweiger Michal R MR

BMC medical genomics 20110929

<h4>Background</h4>Massively parallel sequencing technologies have brought an enormous increase in sequencing throughput. However, these technologies need to be further improved with regard to reproducibility and applicability to clinical samples and settings.<h4>Methods</h4>Using identification of genetic variations in prostate cancer as an example we address three crucial challenges in the field of targeted re-sequencing: Small nucleotide variation (SNV) detection in samples of formalin-fixed ...[more]

PMID: 21958464

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